Pipeline illustration of Eclipse 2.0: Pseudohyphal growth (%) is calculated using the number of pixels in pseudohyphae segments divided by the number of pixels in the main colony segment.
Pseudohyphal growth (%) from left to right: 24.76 %, 48.25 %, 95.10 %
Descriptions
Phenotyping Approach:
PSH was screened using solid agar with synthetic low-ammonium dextrose (SLAD) 2X medium (2% dextrose, 2% agar, 0.34% yeast nitrogen base without amino acids or ammonium sulfate, and 50 µl of 1M ammonium sulfate) in OmniTrays to create a nitrogen-limiting condition. The SLAD plates were imaged after 5 days of 30 C incubation using an Epson Expression 11000 XL scanner. Each image was split into 96 images for further analysis.
PSH was quantified using a computational pipeline: Eclipse 2.0.